![]() ![]() d– f, scatter plots of d orientation preference, e orientation tuning width and f orientation selectivity index (OSI) from von-Mises fitted tuning curves of each neuron before and 5 days after ablation. ![]() c Polar plots depicting un-normalized orientation-tuning curves at each time point. b Peak-normalized orientation-tuning curves of the example neuron at D0 (black), D1 (red), D3 (purple), D5 (blue) and overlay of D0–5. Right insets: Zoomed-in view of the neuron’s responses to a rightward moving grating in 30 trials at D0 (blue dot) and D5 (red dot). Stimulus onset and offset are marked with broken yellow lines. Each column depicts responses to 12 directions (far left) before (D0) and 1 (D1), 3 (D3), and 5 days (D5) after ablation. Bottom: Heat map depictions of the neuron’s single trial somatic calcium responses. a Top: 3D reconstruction of an example neuron before and after apical dendrite ablation. Orientation-tuning functions are unchanged following apical dendrite ablation. ![]() In conclusion, neuronal orientation-tuning appears remarkably robust to loss of dendritic input. Computational modeling corroborated our results and put limits on how orientation preferences among basal dendrites differ in order to reproduce the post-ablation data. Furthermore, orientation-tuning curves were remarkably robust to the removal of basal dendrites: ablation of 2 basal dendrites was needed to cause a small shift in orientation preference, without significantly altering tuning width. We found that removing the apical dendritic tuft did not alter orientation-tuning. We performed 2-photon dendritic micro-dissection on layer-2/3 pyramidal neurons in mouse primary visual cortex. However, how a neuron's dendritic domains relate to its functional selectivity has not been demonstrated experimentally. It has been proposed that feed-forward inputs to basal dendrites drive a neuron's stimulus preference, while feedback inputs to apical dendrites sharpen selectivity. ![]() neurons integrate synaptic inputs from basal and apical dendrites to generate stimulus-specific responses. 9 Brigham and Women's Hospital and Jamaica Plain VA Hospital, Harvard Medical School, Boston, MA, USA.
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